Visualization in freeze-dried tissue sections of structures to be studied by quantitative histochemistry.

B. Hellman, A. Lernmark

Research output: Contribution to journalArticlepeer-review

Abstract

The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic β cells.
Original languageEnglish
Pages (from-to)186-191
JournalThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Volume19
Issue number3
DOIs
Publication statusPublished - 1971 Jan 1
Externally publishedYes

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