Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.

Edgar Pera, Helena Acosta, Nadège Gouignard, Maria Climent

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.
Original languageEnglish
Title of host publicationIn Situ Hybridization Methods
EditorsGiselbert Hauptmannn
Place of PublicationNew York
PublisherHumana Press
Pages151-167
Edition1
ISBN (Electronic)978-1-4939-2303-8
ISBN (Print)978-1-4939-2302-1, 978-1-4939-4463-7
DOIs
Publication statusPublished - 2015

Publication series

NameNeuromethods
PublisherHumana Press, New York, NY
Volume99
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Subject classification (UKÄ)

  • Neurosciences

Free keywords

  • Xenopus , Whole-mount in situ hybridization , Immunohistochemistry , pH3 , TUNEL assay

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