Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.

Edgar Pera; Helena Acosta; Nadège Gouignard; Maria Climent

doi:10.1007/978-1-4939-2303-8

Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.

Edgar Pera, Helena Acosta, Nadège Gouignard, Maria Climent

Stem Cell Center

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.

Original language	English
Title of host publication	In Situ Hybridization Methods
Editors/authors	Giselbert Hauptmannn
Place of Publication	New York
Publisher	Humana Press
Pages	151-167
Edition	1
ISBN (Electronic)	978-1-4939-2303-8
ISBN (Print)	978-1-4939-2302-1, 978-1-4939-4463-7
DOIs	https://doi.org/10.1007/978-1-4939-2303-8
Publication status	Published - 2015

Publication series

Name	Neuromethods
Publisher	Humana Press, New York, NY
Volume	99
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Subject classification (UKÄ)

Neurosciences

Free keywords

Xenopus , Whole-mount in situ hybridization , Immunohistochemistry , pH3 , TUNEL assay

Access to Document

10.1007/978-1-4939-2303-8

Cite this

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abstract = "Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.",

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Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos. / Pera, Edgar; Acosta, Helena; Gouignard, Nadège et al.
In Situ Hybridization Methods. ed. / Giselbert Hauptmannn. 1. ed. New York: Humana Press, 2015. p. 151-167 (Neuromethods; Vol. 99).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

TY - CHAP

T1 - Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.

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AU - Acosta, Helena

AU - Gouignard, Nadège

AU - Climent, Maria

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Y1 - 2015

N2 - Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.

AB - Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.

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M3 - Book chapter

SN - 978-1-4939-2302-1

SN - 978-1-4939-4463-7

T3 - Neuromethods

SP - 151

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BT - In Situ Hybridization Methods

A2 - Hauptmannn, Giselbert

PB - Humana Press

CY - New York

ER -

Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.

Abstract

Publication series

UN SDGs

Subject classification (UKÄ)

Free keywords

Access to Document

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Cite this