Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs.

Karin Warfvinge, Philip H Schwartz, Jens Folke Kiilgaard, Morten la Cour, Michael J Young, Erik Scherfig, Henry Klassen

Research output: Contribution to journalArticlepeer-review

273 Downloads (Pure)

Abstract

To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.
Original languageEnglish
Article number948740
JournalJournal of transplantation
Volume2011
Issue numberArticle ID 948740
DOIs
Publication statusPublished - 2011

Subject classification (UKÄ)

  • Surgery

Fingerprint

Dive into the research topics of 'Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs.'. Together they form a unique fingerprint.

Cite this