Flow Cytometry Core Facility at LUDC -

Flow Cytometry Core Facility at LUDC

Infrastructure

Research areas and keywords

UKÄ subject classification

  • Medical and Health Sciences

Type of infrastructure

  • Equipment
  • Services

Description

The Flow Cytometry Core Facility at LUDC provides instrumentation and technical assistance to perform analysis using flow cytometry and operator based cell sorting service to all Lund University scientists as well as scientists from other institutes and industry.

Our facility houses one state-of-the-art cell sorter, two analysers and one multiplex analyser. Find more details on the facility's webpage:
https://www.ludc.lu.se/resources/flow-cytometry-core-facility-at-ludc

Equipment and resources

For more details please visit: Cell sorter
https://www.ludc.lu.se/resources/flow-cytometry-core-facility/instruments

Cell sorter

BD FACS Aria Fusion
4 lasers (405, 488, 561, 640 nm)
16 fluorescence parameters
Acquisition tubes: 1.5, 5, or 15 ml tubes
Collection: 1.5, 2, 5 or 15 ml tubes or 6-, 12, 24-l, 48-, 96- or 384-well plates
Bulk sorts up to 4 population simultanesouly or single cell sorts
Temperature controlled options


Cell Analyzers

CytoFLEX

3 lasers (405, 488, 640 nm)
13 fluorescence parameters
Acqusition tubes: 1.5 or 5 ml tubes
High throughput 96-well plate unit


Gallios

3 lasers (405, 488, 638 nm)
10 fluorescence parameters
Acquisition tubes: 5 ml tubes
32 tube Multi Carousel Loader (MCL)


Luminex

Up to 100 analyts simultaneously
Very small sample volume
High throughput 96-well plate unit

Services provided

The facility provides:

Access to state-of-the-art cell analyser
Operator based cell sorting service
On site training for the cell analyzer
Technical support
Assistance with experimental design, data anayzer and interpretation
Assistance in developing novel ulticolor flow cytometric techniques
Performing of flow cytometric experiments

Management of the infrastructure

Head of facility and point of contact:
Monika Dudenhöffer-Pfeifer, PhD
Associate Researcher

Bookings and Cancellations

Cell Sorter
For cell sorting request, please, contact the head of the facility.

Cell Analysers
Approved users can book the different instruments in the Flow Cytometer Core Facility online calendar.

In order to get an approved user it is very important to have a basic knowledge in flow cytometry. Appropriate literature or links can be found under ´links´. With a good basci knowledge the user can book an instruction session via email followed by an on site training with users´s samples.

Cancellation should be done at least 24 hours prior the booking.

Available for loan

Available for loan - internal and external

Terms of access:

Monika Dudenhöffer-Pfeifer, PhD

E-mail
monika.dudenhoffer-pfeifer@med.lu.se

Address
Lund University
Clinical Research Centre
Jan Waldenströms gata 35
Rooms; 91-10-051 (Facility), 91-12-059 (Office)
214 28 Malmö