High-resolution fluorescence microscopy

Infrastructure

Research areas and keywords

UKÄ subject classification

  • Physical Chemistry

Type of infrastructure

  • Equipment

Description

Two custom made setups for high-resolution fluorescence imaging capable of single molecule studies, fluorescence recovery after photobleaching (FRAP) and total internal reflection fluorescence (TIRF) imaging at different wavelengths (both 488 nm and 638 nm excitation). One of the setups is combined with a piezo-controlled (with nm resolution) holder for nano- and micropipette manipulation and delivery.

Equipment and resources

1) Setup based around a Nikon Eclipse TE2000 U microscope, with a Nikon Apo TIRF 60x (NA = 1.49) oil objective for the TIRF studies. Other objectives available are: 10x and 20x air objectives. Excitation light comes from two Cobolt MLD diode lasers operating at 488 nm (maximum 60 mW power) and 638 nm (maximum 140 mW power). The excitation laser power is adjusted with a motorized filter wheel from Thorlabs. Images are collected with a sCMOS camera from Hamamatsu (ORCA-Flash4.0 LT).

2) Setup based around a Nikon Eclipse Ti-E microscope, with a Nikon Apo TIRF 100x (NA = 1.49) oil objective. Excitation light comes from either of four Oxxius diode lasers operating at 405 nm (100 mW power), 488 nm (100 mW power), 561 (100 mW power) and 638 nm (180 mW power). Images are collected with a sCMOS camera from Photometrics (Prime 95B). The setup supports perfect focus imaging and has a motorized stage for automation.

Available for loan

Not available for loan

Terms of access:

Access via collaboration