MultiPark Flow Cytometry Facility

Infrastructure

Research areas and keywords

UKÄ subject classification

  • Medical and Health Sciences

Type of infrastructure

  • Equipment
  • Services

Description

Flow cytometry analysis and sorting – FACS
Using flow cytometry you may measure fluorescence intensity of particles (cells) in a liquid stream. Analysis of live and fixed cells based on size, granularity and fluorescent markers.
Fluorescent targets include
• Fluorescent proteins
• Cell surface antigen expression
• Intracellular antigen expression
• DNA content
Fluorescence Activated Cell Sorting (FACS)
• Physically separating out cell populations based on the fluorescence labeling and light scattering properties.

Equipment and resources

BD FACS Aria III
Fluorescence activated cell sorter (FACS)
• 3 LASERs: 488 nm (blue) 561 m (yellow-green), 640 nm (red)
• Detection of 2 scatter channels and 10 fluorescent channels
• cell sorting and analysis
• Sort 1-4 populations simultaneously into tubes
• Plate sorting of populations or single cells
e.g. 384, 96, 60, 48, 24, 6, 4 well fomat, PCR-strip
• Sorting on to slides

BD Accuri C6
Flow cytometry analysis instrument
• 2 LASERs: 488 nm (blue) 640 nm (red)
• Detection of 2 scatter channels and 4 fluorescent channels
• easy to learn and simple to use

Services provided

Services include assistance with:
• Cell Sorting
• Data acquisition and data analysis
• Advice for experiments and data analysis
• Participating in developing new protocols and techniques
• FlowJo Analysis
Training
• One-to-one hands on training
• Theoretic and practical workshops

Management of the infrastructure

MultiPark

Available for loan

Available for loan - internal and external

Terms of access:

Trained, independent, internal users get access to the equipment. <br/>Users who need assistance and external users have access during the facility personnel working hours<br/>Independent users have access to a booking calendar<br/>Users that require assistance book via e-mail Anna.Hammarberg@med.lu.se<br/><br/>All sample preparation should be done by the user.