Vector Core -

Stem Therapy Viral vector core facility

Infrastructure

Research areas and keywords

UKÄ subject classification

  • Cell and Molecular Biology

Description

Objectives and background: The Vector Core Facility is part of the strategic research area StemTherapy. The main purpose of the Vector core is to provide investigators the scientific resources necessary to produce state-of-the-art recombinant oncoretroviral and lentiviral vectors for use in gene transfer experiments. The Vector core facility maintains a P2 facility with the proper expertise, equipment and permissions to produce the vectors. Technichal platform for vector production: An investigator or laboratory that wants to have an oncoretroviral or lentiviral vector made can send the vector plasmid with the gene of interest to the Vector unit. Through consultation the investigator can be given advice about promoter choice and vector design prior to his or her constructing the viral vector plasmid. The core also provides cloning services to its users.

Equipment and resources

Cell culture lab, ultracentrifuge and rotors

Digital and physical collections

Records of all orders completed, datafile with all over 600 vectors at the BMC for faciltiating sharing collaboration with other users.

Services provided

high titer vector production, titration (FACS QPCR), cloning consultation, cloning service

Management of the infrastructure

Niels-Bjarne Woods (director) Beata Linqvist (100% manager/technicain viral production) and Xiaojie Xian (75% manager/technician cloning)

Available for loan

Available for loan - internal and external

Terms of access:

Open access, predominantly used by Stem therapy users and also Stem Cell Center users.