Two-Photon Microscopy (in vivo / ex vivo)

Infrastructure

Research areas and keywords

UKÄ subject classification

  • Medical and Health Sciences

Type of infrastructure

  • Equipment
  • Services

Description

Two-photon microscopy is the method of choice for imaging living cells and tissues (intravital microscopy). It allows imaging with very high temporal and spatial resolution in thick biological material. The ‘in vivo’ setup can be used to image the processes in the living mouse, i.e. superficial layers of the cerebral cortex. The ‘ex vivo’ system is designed to image thick live preparations, such as brain regions that are not accessible to ‘in vivo’ microscopy. In this setup, two-photon imaging can also be combined with patch-clamp electrophysiological recordings. Although primarily acquired to perform studies in neuroscience, the system is available to all interested researchers at and outside LU.

Both microscopes are paired with accessory setups, such as stereotactic frames for the ‘in vivo’ part and an additional room for samples preparation for the ‘ex vivo’ set up. For more details, contact the responsible persons.

Equipment and resources

The core of each system is a ZEISS LSM710 NLO microscope, connected to a MaiTai two photon laser. The ‘ex vivo’ system is combined with a standard patch clamp rig (Axon amplifier and Digidata digitizer).

Services provided

We provide training of new users and also assistance with experiments if necessary, for a small fee.

Management of the infrastructure

The two photon microscopes are currently managed by Karsten Ruscher and Tim Fieblinger (see below), who also admit and train user.

Available for loan

Available for loan - internal and external

Terms of access:

The admission works in three steps: 1) Contact and Consultation 2) Introduction and Pilot 3) Access and Assistance. For more information, visit the web page.