Analyzing exhaled particles for detecting airway specific biomarkers

Project: Dissertation

Description

Background: The technique of sampling exhaled air is attractive because it is noninvasive and so allows repeated sampling with ease and no risk for the patient. Knowledge of the biomarkers' origin is important to correctly understand and interpret the data. Endogenous particles, formed in the airways, are exhaled and reflect chemical composition of the respiratory tract lining fluid (RTLF).
A new non-invasive method, PExA, is suitable for studying the composition of the RTLF. As we breathe small particles of liquid are formed from the RTLF. These particles follow the airway during exhalation and can be sample of RTLF can thus be obtained by sampling these particles. The PExA method is a non-invasive method to measuring proteins and lipids as biomarkers in RTLF collected from the small airways. Exhaled particle number concentrations in the size interval 0.30-2.0 micron are recorded PExA method and biomarker collection: A non-invasive method for studying the composition of the RTLF. As
we breathe small particles of liquid are formed from the RTLF. These particles follow the airway during exhalation and can be sample of RTLF can thus be obtained by sampling these particles. The PExA method is a
non-invasive method to measuring proteins and lipids as biomarkers in RTLF collected from the small airways.
Exhaled particle number concentrations in the size interval 0.30-2.0 microm are recorded. Samples and analysis is thus made of endogenous particles from breath. Collection can thus be made when the patient is on a ventilator.
In a recent published study the PExA method was used to sample surfactant protein-A (SP-A) in patients who underwent lung transplantation. The recipients who developed cronic rejection had significant low levels of SP-A compared to the recipient who did not develop cronic rejection, and also compared to healthy subjects We set out to investigate the composition of biomarkers in exhaled air n patients who underwent lungtransplantation but also patients with lungcancer.
Aim: Our aim is to find a biomarker among exhaled particles which can be strongly connected patients with lungcancer but also to cronic rejection among patients who underwent lungtransplantation, and in lungs evaluated in ex vivo lung perfusion for lungtransplantation but also in patients with different kind of
lungcancer
StatusNot started

Participants