17β-estradiol regulates the expression of apolipoprotein M through estrogen receptor α-specific binding motif in its promoter

Research output: Contribution to journalArticle

Abstract

Background: We have previously demonstrated that estrogen could significantly enhance expression of apolipoprotein M (apoM), whereas the molecular basis of its mechanism is not fully elucidated yet. To further investigate the mechanism behind the estrogen induced up-regulation of apoM expression. Results: Our results demonstrated either free 17β-estradiol (E2) or membrane-impermeable bovine serum albumin-conjugated E2 (E2-BSA) could modulate human apoM gene expression via the estrogen receptor alpha (ER-α) pathway in the HepG2 cells. Moreover, experiments with the luciferase activity analysis of truncated apoM promoters could demonstrate that a regulatory region (from-1580 to −1575 bp (−GGTCA-)) upstream of the transcriptional start site of apoM gene was essential for the basal transcriptional activity that regulated by the ER-α. With the applications of an electrophoresis mobility shift assay and a chromatin immunoprecipitation assay, we could successfully identify a specific ER-α binding element in the apoM promoter region. Conculsion: In summary, the present study indicates that 17β-estradiol induced up-regulation of apoM in HepG2 cells is through an ER-α-dependent pathway involving ER-α binding element in the promoter of the apoM gene.

Details

Authors
  • Jiang Wei
  • Yang Yu
  • Guang-Hua Luo
  • Yue hua Feng
  • Yuan-ping Shi
  • Jun Zhang
  • Qin feng Mu
  • Miao mei Yu
  • Li li Pan
  • Maria Berggren-Söderlund
  • Peter Nilsson-Ehle
  • Xiao-Ying Zhang
  • Ning Xu
Organisations
External organisations
  • Third Affiliated Hospital of Soochow University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Physiology
  • Medicinal Chemistry

Keywords

  • Apolipoprotein M, Chromatin Immunoprecipitation (ChIP) assay, Electrophoretic mobility shift assay (EMSA), Estrogen receptor alpha, Estrogen responsive element
Original languageEnglish
Article number66
Pages (from-to)1-8
JournalLipids in Health and Disease
Volume16
Issue number1
Publication statusPublished - 2017 Mar 31
Publication categoryResearch
Peer-reviewedYes