3D MAS NMR Experiment Utilizing Through-Space 15N-15N Correlations

Research output: Contribution to journalArticle

Abstract

We demonstrate a novel 3D NNC magic angle spinning NMR experiment that generates 15N-15N internuclear contacts in protein systems using an optimized 15N-15N proton assisted recoupling (PAR) mixing period and a 13C dimension for improved resolution. The optimized PAR condition permits the acquisition of high signal-to-noise 3D data that enables backbone chemical shift assignments using a strategy that is complementary to current schemes. The spectra can also provide distance constraints. The utility of the experiment is demonstrated on an M01-42 fibril sample that yields high-quality data that is readily assigned and interpreted. The 3D NNC experiment therefore provides a powerful platform for solid-state protein studies and is broadly applicable to a variety of systems and experimental conditions.

Details

Authors
  • Kevin J. Donovan
  • Robert Silvers
  • Sara Linse
  • Robert G Griffin
Organisations
External organisations
  • Massachusetts Institute of Technology
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology
Original languageEnglish
Pages (from-to)6518-6521
Number of pages4
JournalJournal of the American Chemical Society
Volume139
Issue number19
Publication statusPublished - 2017 May 17
Publication categoryResearch
Peer-reviewedYes