3He MRI-based assessment of posture-dependent regional ventilation gradients in rats

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3He MRI-based assessment of posture-dependent regional ventilation gradients in rats. / Månsson, Sven; Deninger, Anselm J; Magnusson, Peter; Pettersson, Göran; Olsson, Lars E; Hansson, Georg; Wollmer, Per; Golman, Klaes.

In: Journal of Applied Physiology, Vol. 98, No. 6, 06.2005, p. 2259-67.

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Månsson, Sven ; Deninger, Anselm J ; Magnusson, Peter ; Pettersson, Göran ; Olsson, Lars E ; Hansson, Georg ; Wollmer, Per ; Golman, Klaes. / 3He MRI-based assessment of posture-dependent regional ventilation gradients in rats. In: Journal of Applied Physiology. 2005 ; Vol. 98, No. 6. pp. 2259-67.

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TY - JOUR

T1 - 3He MRI-based assessment of posture-dependent regional ventilation gradients in rats

AU - Månsson, Sven

AU - Deninger, Anselm J

AU - Magnusson, Peter

AU - Pettersson, Göran

AU - Olsson, Lars E

AU - Hansson, Georg

AU - Wollmer, Per

AU - Golman, Klaes

PY - 2005/6

Y1 - 2005/6

N2 - A recently developed method for quantitative assessment of regional lung ventilation was employed for the study of posture-dependent ventilation differences in rats. The measurement employed hyperpolarized (3)He MRI to detect the build-up of the signal intensity after increasing numbers of (3)He breaths, which allowed for computation of a regional ventilation parameter. A group of six anesthetized rats was studied in both supine and prone postures. Three-dimensional maps of the ventilation parameter were obtained with high spatial resolution (voxel volume approximately 2 mm(3)). Vertical (dorsal-ventral) gradients of the ventilation index, defined as the regional ventilation normalized by the average ventilation within the whole lung, were investigated. Variations in the regional distribution of the ventilation parameter, as well as of the ventilation index, could be detected, depending on the posture of the rats. In supine posture, ventilation was elevated in the dependent parts of the lungs, with a linear gradient of the ventilation index of -0.11 +/- 0.03 cm(-1). In prone posture, the distribution of ventilation was more uniform, with a significantly (P < 0.001) smaller gradient of the ventilation index of -0.01 +/- 0.02 cm(-1). It is concluded that the (3)He MRI-based method can detect and quantify regional ventilation gradients in animals as small as the rat and that these gradients depend on prone or supine posture of the animal.

AB - A recently developed method for quantitative assessment of regional lung ventilation was employed for the study of posture-dependent ventilation differences in rats. The measurement employed hyperpolarized (3)He MRI to detect the build-up of the signal intensity after increasing numbers of (3)He breaths, which allowed for computation of a regional ventilation parameter. A group of six anesthetized rats was studied in both supine and prone postures. Three-dimensional maps of the ventilation parameter were obtained with high spatial resolution (voxel volume approximately 2 mm(3)). Vertical (dorsal-ventral) gradients of the ventilation index, defined as the regional ventilation normalized by the average ventilation within the whole lung, were investigated. Variations in the regional distribution of the ventilation parameter, as well as of the ventilation index, could be detected, depending on the posture of the rats. In supine posture, ventilation was elevated in the dependent parts of the lungs, with a linear gradient of the ventilation index of -0.11 +/- 0.03 cm(-1). In prone posture, the distribution of ventilation was more uniform, with a significantly (P < 0.001) smaller gradient of the ventilation index of -0.01 +/- 0.02 cm(-1). It is concluded that the (3)He MRI-based method can detect and quantify regional ventilation gradients in animals as small as the rat and that these gradients depend on prone or supine posture of the animal.

KW - Administration, Inhalation

KW - Algorithms

KW - Animals

KW - Contrast Media

KW - Helium

KW - Image Interpretation, Computer-Assisted

KW - Imaging, Three-Dimensional

KW - Isotopes

KW - Lung

KW - Magnetic Resonance Imaging

KW - Men

KW - Posture

KW - Prone Position

KW - Pulmonary Ventilation

KW - Rats

KW - Rats, Sprague-Dawley

KW - Supine Position

U2 - 10.1152/japplphysiol.00245.2004

DO - 10.1152/japplphysiol.00245.2004

M3 - Article

VL - 98

SP - 2259

EP - 2267

JO - Journal of Applied Physiology

T2 - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 1522-1601

IS - 6

ER -