4.1R-deficient human red blood cells have altered phosphatidylserine exposure pathways and are deficient in CD44 and CD47 glycoproteins

Research output: Contribution to journalArticle

Abstract

Background Protein 4.1R is an important component of die red cell membrane skeleton. It imparts structural integrity and has transmembrane signaling roles by direct interactions with transmembrane proteins and other membrane skeletal components, notably p55 and calmodulin. Design and Methods Spontaneous and ligation-induced phosphatidylserine exposure on erythrocytes from two patients with 4.1R deficiency were studied, using CD47 glycoprotein and glycophorin C as ligands We also looked for protein abnormalities in the 4.1R - based multiprotein complex. Results Phosphatidylserine exposure was significantly increased in 4.1R-deficient erythrocytes obtained from the two different individuals when ligands to CD47 glycoprotein were bound. Spontaneous phosphatidylserine exposure was normal. 4.1R, glycophorin C and p55 were missing or sharply reduced. Furthermore there was an alteration or deficiency of CD47 glycoprotein and a lack of CD44 glycoprotein. Based on a recent study in 4.1R-deficient mice, we found that there are clear functional differences between interactions of human red cell 4.1R and its murine counterpart Conclusions Glycophorin C is known to bind 4.1R, and we have defined previously that it also binds CD47 From our evidence, we suggest that 4.1R plays a role in the phosphatidylserine exposure signaling pathway that is of Fundamental importance in red cell turnover The linkage of CD44 to 4.1R may be relevant to this process

Details

Authors
  • Kris P. Jeremy
  • Zoe E. Plummer
  • David J. Head
  • Tracey E. Madgett
  • Kelly L. Sanders
  • Amanda Wallington
  • Jill Storry
  • Florinda Gilsanz
  • Jean Delaunay
  • Neil D. Avent
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology

Keywords

  • glycophorin C, CD47, CD44, phosphatidylserine, 4.1R deficiency
Original languageEnglish
Pages (from-to)1354-1361
JournalHaematologica
Volume94
Issue number10
Publication statusPublished - 2009
Publication categoryResearch
Peer-reviewedYes