A circular RNA generated from an intron of the insulin gene controls insulin secretion

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A circular RNA generated from an intron of the insulin gene controls insulin secretion. / Stoll, Lisa; Rodríguez-Trejo, Adriana; Guay, Claudiane; Brozzi, Flora; Bayazit, Mustafa Bilal; Gattesco, Sonia; Menoud, Véronique; Sobel, Jonathan; Marques, Ana Claudia; Venø, Morten Trillingsgaard; Esguerra, Jonathan Lou S.; Barghouth, Mohammad; Suleiman, Mara; Marselli, Lorella; Kjems, Jørgen; Eliasson, Lena; Renström, Erik; Bouzakri, Karim; Pinget, Michel; Marchetti, Piero; Regazzi, Romano.

In: Nature Communications, Vol. 11, No. 1, 5611, 2020.

Research output: Contribution to journalArticle

Harvard

Stoll, L, Rodríguez-Trejo, A, Guay, C, Brozzi, F, Bayazit, MB, Gattesco, S, Menoud, V, Sobel, J, Marques, AC, Venø, MT, Esguerra, JLS, Barghouth, M, Suleiman, M, Marselli, L, Kjems, J, Eliasson, L, Renström, E, Bouzakri, K, Pinget, M, Marchetti, P & Regazzi, R 2020, 'A circular RNA generated from an intron of the insulin gene controls insulin secretion', Nature Communications, vol. 11, no. 1, 5611. https://doi.org/10.1038/s41467-020-19381-w

APA

Stoll, L., Rodríguez-Trejo, A., Guay, C., Brozzi, F., Bayazit, M. B., Gattesco, S., Menoud, V., Sobel, J., Marques, A. C., Venø, M. T., Esguerra, J. L. S., Barghouth, M., Suleiman, M., Marselli, L., Kjems, J., Eliasson, L., Renström, E., Bouzakri, K., Pinget, M., ... Regazzi, R. (2020). A circular RNA generated from an intron of the insulin gene controls insulin secretion. Nature Communications, 11(1), [5611]. https://doi.org/10.1038/s41467-020-19381-w

CBE

Stoll L, Rodríguez-Trejo A, Guay C, Brozzi F, Bayazit MB, Gattesco S, Menoud V, Sobel J, Marques AC, Venø MT, Esguerra JLS, Barghouth M, Suleiman M, Marselli L, Kjems J, Eliasson L, Renström E, Bouzakri K, Pinget M, Marchetti P, Regazzi R. 2020. A circular RNA generated from an intron of the insulin gene controls insulin secretion. Nature Communications. 11(1):Article 5611. https://doi.org/10.1038/s41467-020-19381-w

MLA

Vancouver

Author

Stoll, Lisa ; Rodríguez-Trejo, Adriana ; Guay, Claudiane ; Brozzi, Flora ; Bayazit, Mustafa Bilal ; Gattesco, Sonia ; Menoud, Véronique ; Sobel, Jonathan ; Marques, Ana Claudia ; Venø, Morten Trillingsgaard ; Esguerra, Jonathan Lou S. ; Barghouth, Mohammad ; Suleiman, Mara ; Marselli, Lorella ; Kjems, Jørgen ; Eliasson, Lena ; Renström, Erik ; Bouzakri, Karim ; Pinget, Michel ; Marchetti, Piero ; Regazzi, Romano. / A circular RNA generated from an intron of the insulin gene controls insulin secretion. In: Nature Communications. 2020 ; Vol. 11, No. 1.

RIS

TY - JOUR

T1 - A circular RNA generated from an intron of the insulin gene controls insulin secretion

AU - Stoll, Lisa

AU - Rodríguez-Trejo, Adriana

AU - Guay, Claudiane

AU - Brozzi, Flora

AU - Bayazit, Mustafa Bilal

AU - Gattesco, Sonia

AU - Menoud, Véronique

AU - Sobel, Jonathan

AU - Marques, Ana Claudia

AU - Venø, Morten Trillingsgaard

AU - Esguerra, Jonathan Lou S.

AU - Barghouth, Mohammad

AU - Suleiman, Mara

AU - Marselli, Lorella

AU - Kjems, Jørgen

AU - Eliasson, Lena

AU - Renström, Erik

AU - Bouzakri, Karim

AU - Pinget, Michel

AU - Marchetti, Piero

AU - Regazzi, Romano

PY - 2020

Y1 - 2020

N2 - Fine-tuning of insulin release from pancreatic β-cells is essential to maintain blood glucose homeostasis. Here, we report that insulin secretion is regulated by a circular RNA containing the lariat sequence of the second intron of the insulin gene. Silencing of this intronic circular RNA in pancreatic islets leads to a decrease in the expression of key components of the secretory machinery of β-cells, resulting in impaired glucose- or KCl-induced insulin release and calcium signaling. The effect of the circular RNA is exerted at the transcriptional level and involves an interaction with the RNA-binding protein TAR DNA-binding protein 43 kDa (TDP-43). The level of this circularized intron is reduced in the islets of rodent diabetes models and of type 2 diabetic patients, possibly explaining their impaired secretory capacity. The study of this and other circular RNAs helps understanding β-cell dysfunction under diabetes conditions, and the etiology of this common metabolic disorder.

AB - Fine-tuning of insulin release from pancreatic β-cells is essential to maintain blood glucose homeostasis. Here, we report that insulin secretion is regulated by a circular RNA containing the lariat sequence of the second intron of the insulin gene. Silencing of this intronic circular RNA in pancreatic islets leads to a decrease in the expression of key components of the secretory machinery of β-cells, resulting in impaired glucose- or KCl-induced insulin release and calcium signaling. The effect of the circular RNA is exerted at the transcriptional level and involves an interaction with the RNA-binding protein TAR DNA-binding protein 43 kDa (TDP-43). The level of this circularized intron is reduced in the islets of rodent diabetes models and of type 2 diabetic patients, possibly explaining their impaired secretory capacity. The study of this and other circular RNAs helps understanding β-cell dysfunction under diabetes conditions, and the etiology of this common metabolic disorder.

U2 - 10.1038/s41467-020-19381-w

DO - 10.1038/s41467-020-19381-w

M3 - Article

C2 - 33154349

AN - SCOPUS:85095425491

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 5611

ER -