A Comprehensive Investigation on Common Polymorphisms in the MDR1/ABCB1 Transporter Gene and Susceptibility to Colorectal Cancer

Research output: Contribution to journalArticle

Abstract

ATP Binding Cassette B1 (ABCB1) is a transporter with a broad substrate specificity involved in the elimination of several carcinogens from the gut. Several polymorphic variants within the ABCB1 gene have been reported as modulators of ABCB1-mediated transport. We investigated the impact of ABCB1 genetic variants on colorectal cancer (CRC) risk. A hybrid tagging/functional approach was performed to select 28 single nucleotide polymorphisms (SNPs) that were genotyped in 1,321 Czech subjects, 699 CRC cases and 622 controls. In addition, six potentially functional SNPs were genotyped in 3,662 German subjects, 1,809 cases and 1,853 controls from the DACHS study. We found that three functional SNPs (rs1202168, rs1045642 and rs868755) were associated with CRC risk in the German population. Carriers of the rs1202168_T and rs868755_T alleles had an increased risk for CRC (P-trend = 0.016 and 0.029, respectively), while individuals bearing the rs1045642_C allele showed a decreased risk of CRC (P-trend = 0.022). We sought to replicate the most significant results in an independent case-control study of 3,803 subjects, 2,169 cases and 1,634 controls carried out in the North of Germany. None of the SNPs tested were significantly associated with CRC risk in the replication study. In conclusion, in this study of about 8,800 individuals we show that ABCB1 gene polymorphisms play at best a minor role in the susceptibility to CRC.

Details

Authors
  • Daniele Campa
  • Juan Sainz
  • Barbara Pardini
  • Ludmila Vodickova
  • Alessio Naccarati
  • Anja Rudolph
  • Jan Novotny
  • Stephan Buch
  • Witigo von Schoenfels
  • Clemens Schafmayer
  • Henry Voelzke
  • Michael Hoffmeister
  • Bernd Frank
  • Roberto Barale
  • Jochen Hampe
  • Jenny Chang-Claude
  • Hermann Brenner
  • Pavel Vodicka
  • Federico Canzian
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Public Health, Global Health, Social Medicine and Epidemiology
Original languageEnglish
JournalPLoS ONE
Volume7
Issue number3
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes

Total downloads

No data available