A cross-contamination-free SELEX platform for a multi-target selection strategy

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Abstract

Multi-target aptamer selection, known as multiplex systematic evolution of ligands by exponential enrichment (SELEX), is rapidly drawing interest because of its potential to enable high-speed, high-throughput aptamer selection. The parallelization of chemical processes by integrating microfluidic unit operations is a key strategy for developing a multiplex SELEX process. One of the potential problems with on-chip multiplexing chemical processes is cross-contamination. In order to avoid this, we propose a microfluidic network platform that uses pneumatic valves to allow the serial loading and incubation of aptamers with sol-gel entrapped target proteins. After target binding inside the sol-gels, the cross-contamination-free parallel elution of specifically bound aptamers is performed. The platform allows selective binding with five different targets immobilized in sol-gel spots. When eluting bound species, cross-contamination is avoided by sealing the adjacent elution chambers from each other using the pneumatic microvalves. Consequently, we demonstrate specific aptamer binding to the respective protein target and subsequent aptamer elution without any cross-contamination. This proof of concept opens the way to increased automation and microscale parallel processing of the SELEX methodology.

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Subject classification (UKÄ) – MANDATORY

  • Medical Engineering

Keywords

  • Microfluidic pneumatic valve, Microheater, Multiplex SELEX, Sol-gel, immobilization, Aptamer
Original languageEnglish
Pages (from-to)38-45
JournalBiochip Journal
Volume7
Issue number1
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes