A fast semi-quantitative LC-MS method for measurement of intact apolipoprotein A-I reveals novel proteoforms in serum.

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Abstract

Surrogate markers for reverse cholesterol transport (RCT) efficiency such as HDL cholesterol and immune methods for apolipoprotein A-I (ApoA-I) may not fully reflect the actual efficiency of the RCT pathway. Several genetic variants and different posttranslational proteoforms of ApoA-I may unevenly affect the functionality of the HDL particle to efflux cholesterol. A method employing top-down immunoaffinity LC-MS of ApoA-I in order to characterize the most prevalent ApoA-I proteoforms in human plasma is described.

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Subject classification (UKÄ) – MANDATORY

  • Clinical Laboratory Medicine
Original languageEnglish
Pages (from-to)87-95
JournalClinica Chimica Acta
Volume442
Issue numberJan 17
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes