A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction

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A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction. / Liden, A; Karlstrom, A; Lannergard, J; Kalamajski, Sebastian; Guss, B; Rubin, K; Ryden, C.

In: Biochemical and Biophysical Research Communications, Vol. 340, No. 2, 2006, p. 604-610.

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T1 - A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction

AU - Liden, A

AU - Karlstrom, A

AU - Lannergard, J

AU - Kalamajski, Sebastian

AU - Guss, B

AU - Rubin, K

AU - Ryden, C

PY - 2006

Y1 - 2006

N2 - The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemieus was investigated as to effects on murine cell interactions with extracellular matrix proteins, FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alpha V beta 3. C2C12 cells lack collagen-binding beta 1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alpha V beta 3-inhibitor suggested that FNZN promoted contraction by it process requiring alpha V beta 3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alpha V beta 3 integrin, Since alpha V beta 3-mediated contractile events function to counteract edema formation during inflammation. it is possible that FNZN and its secreted homologue FINE modulate edema responses in infected tissues. (c) 2005 Elsevier Inc. All rights reserved.

AB - The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemieus was investigated as to effects on murine cell interactions with extracellular matrix proteins, FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alpha V beta 3. C2C12 cells lack collagen-binding beta 1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alpha V beta 3-inhibitor suggested that FNZN promoted contraction by it process requiring alpha V beta 3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alpha V beta 3 integrin, Since alpha V beta 3-mediated contractile events function to counteract edema formation during inflammation. it is possible that FNZN and its secreted homologue FINE modulate edema responses in infected tissues. (c) 2005 Elsevier Inc. All rights reserved.

KW - infection

KW - adhesion

KW - edema

KW - collagen structure

KW - collagen-binding

KW - integrins

U2 - 10.1016/j.bbrc.2005.12.043

DO - 10.1016/j.bbrc.2005.12.043

M3 - Article

VL - 340

SP - 604

EP - 610

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 1090-2104

IS - 2

ER -