A global network of transcription factors, involving E2A, EBF1 and Foxo1, that orchestrates B cell fate

Research output: Contribution to journalArticle

Abstract

It is now established that the transcription factors E2A, EBF1 and Foxo1 have critical roles in B cell development. Here we show that E2A and EBF1 bound regulatory elements present in the Foxo1 locus. E2A and EBF1, as well as E2A and Foxo1, in turn, were wired together by a vast spectrum of cis-regulatory sequences. These associations were dynamic during developmental progression. Occupancy by the E2A isoform E47 directly resulted in greater abundance, as well as a pattern of monomethylation of histone H3 at lysine 4 (H3K4) across putative enhancer regions. Finally, we divided the pro-B cell epigenome into clusters of loci with occupancy by E2A, EBF and Foxo1. From this analysis we constructed a global network consisting of transcriptional regulators, signaling and survival factors that we propose orchestrates B cell fate.

Details

Authors
  • Yin C. Lin
  • Suchit Jhunjhunwala
  • Christopher Benner
  • Sven Heinz
  • Eva Welinder
  • Robert Mansson
  • Mikael Sigvardsson
  • James Hagman
  • Celso A. Espinoza
  • Janusz Dutkowski
  • Trey Ideker
  • Christopher K. Glass
  • Cornelis Murre
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Rheumatology and Autoimmunity
Original languageEnglish
Pages (from-to)635-U109
JournalNature Immunology
Volume11
Issue number7
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)