A model for the photosystem II reaction center core including the structure of the primary donor P-680

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Abstract

For a detailed understanding of the function of photosystem II (PSII), a molecular structure is needed, The crystal structure has not yet been determined, but the PSII reaction center proteins D1 and D2 show homology with the L and M subunits of the photosynthetic reaction center from purple bacteria, We have modeled important parts of the D1 and D2 proteins on the basis of the crystallographic structure of The reaction center from Rhodopseudomonas viridis. The model contains the central core of the PSII reaction center, including the protein regions for the transmembrane helices B, C, D, and E and loops B-C and C-D connecting the helices, In the model, four chlorophylls, two pheophytins, and the nonheme Fe2+ ion are included. We have applied techniques from computational chemistry that incorporate statistical data on side-chain rotameric states from known protein structures and that describe interactions within the model using an empirical potential energy function. The conformation of chlorophyll pigments in the model was optimized by using exciton interaction calculations in combination with potential energy calculations to rind a solution that agrees with experimentally determined exciton interaction energies, The model is analyzed and compared with experimental results for the regions of Pb-680, the redox active pheophytin, the acceptor side Fe2+, and the tyrosyl radicals Tyr(D) and Tyr(Z), P-680 is proposed to be a weakly coupled chlorophyll a pair which makes three hydrogen bonds with residues on the D1 and D2 proteins. In the model the redox-active pheophytin is hydrogen bonded to D1-Glu130 and possibly also to D1-Tyr126 and D1-Tyr147. Tyr(D) is hydrogen bonded to D2-His190 and also interacts with D2-Gln165. Tyr(Z) is bound in a hydrophilic environment which is partially constituted by D1-Gln165, D1-Asp170, D1-Glu189, and D1-His190, These polar residues are most likely involved in proton transfer from oxidized Tyr(Z) or in metal binding.

Details

Authors
  • B Svensson
  • C Etchebest
  • P Tuffery
  • Paul van Kan
  • J Smith
  • Stenbjörn Styring
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • PHOTOSYNTHETIC REACTION-CENTER, ELECTRON-PARAMAGNETIC-RES, SITE-DIRECTED MUTANTS, NON-HEME IRON, RHODOBACTER-SPHAEROIDES R-26, ENVELOPE MODULATION ESEEM, SYNECHOCYSTIS SP PCC-6803, HERBICIDE BINDING NICHE, REACTION-CENTER COMPLEX, AMINO-ACID SEQUENCE
Original languageEnglish
Pages (from-to)14486-14502
JournalBiochemistry
Volume35
Issue number46
Publication statusPublished - 1996
Publication categoryResearch
Peer-reviewedYes