A molecular map of murine lymph node blood vascular endothelium at single cell resolution

Research output: Contribution to journalArticle


title = "A molecular map of murine lymph node blood vascular endothelium at single cell resolution",
abstract = "Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.",
author = "Kevin Brulois and Anusha Rajaraman and Agata Szade and Sofia Nordling and Ania Bogoslowski and Denis Dermadi and Milladur Rahman and Helena Kiefel and Edward O{\textquoteright}Hara and Koning, {Jasper J.} and Hiroto Kawashima and Bin Zhou and Dietmar Vestweber and Kristy Red-Horse and Mebius, {Reina E.} and Adams, {Ralf H.} and Paul Kubes and Junliang Pan and Butcher, {Eugene C.}",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41467-020-17291-5",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",