A molecular map of murine lymph node blood vascular endothelium at single cell resolution

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A molecular map of murine lymph node blood vascular endothelium at single cell resolution. / Brulois, Kevin; Rajaraman, Anusha; Szade, Agata; Nordling, Sofia; Bogoslowski, Ania; Dermadi, Denis; Rahman, Milladur; Kiefel, Helena; O’Hara, Edward; Koning, Jasper J.; Kawashima, Hiroto; Zhou, Bin; Vestweber, Dietmar; Red-Horse, Kristy; Mebius, Reina E.; Adams, Ralf H.; Kubes, Paul; Pan, Junliang; Butcher, Eugene C.

In: Nature Communications, Vol. 11, No. 1, 3798, 01.12.2020.

Research output: Contribution to journalArticle

Harvard

Brulois, K, Rajaraman, A, Szade, A, Nordling, S, Bogoslowski, A, Dermadi, D, Rahman, M, Kiefel, H, O’Hara, E, Koning, JJ, Kawashima, H, Zhou, B, Vestweber, D, Red-Horse, K, Mebius, RE, Adams, RH, Kubes, P, Pan, J & Butcher, EC 2020, 'A molecular map of murine lymph node blood vascular endothelium at single cell resolution', Nature Communications, vol. 11, no. 1, 3798. https://doi.org/10.1038/s41467-020-17291-5

APA

Brulois, K., Rajaraman, A., Szade, A., Nordling, S., Bogoslowski, A., Dermadi, D., Rahman, M., Kiefel, H., O’Hara, E., Koning, J. J., Kawashima, H., Zhou, B., Vestweber, D., Red-Horse, K., Mebius, R. E., Adams, R. H., Kubes, P., Pan, J., & Butcher, E. C. (2020). A molecular map of murine lymph node blood vascular endothelium at single cell resolution. Nature Communications, 11(1), [3798]. https://doi.org/10.1038/s41467-020-17291-5

CBE

Brulois K, Rajaraman A, Szade A, Nordling S, Bogoslowski A, Dermadi D, Rahman M, Kiefel H, O’Hara E, Koning JJ, Kawashima H, Zhou B, Vestweber D, Red-Horse K, Mebius RE, Adams RH, Kubes P, Pan J, Butcher EC. 2020. A molecular map of murine lymph node blood vascular endothelium at single cell resolution. Nature Communications. 11(1):Article 3798. https://doi.org/10.1038/s41467-020-17291-5

MLA

Vancouver

Brulois K, Rajaraman A, Szade A, Nordling S, Bogoslowski A, Dermadi D et al. A molecular map of murine lymph node blood vascular endothelium at single cell resolution. Nature Communications. 2020 Dec 1;11(1). 3798. https://doi.org/10.1038/s41467-020-17291-5

Author

Brulois, Kevin ; Rajaraman, Anusha ; Szade, Agata ; Nordling, Sofia ; Bogoslowski, Ania ; Dermadi, Denis ; Rahman, Milladur ; Kiefel, Helena ; O’Hara, Edward ; Koning, Jasper J. ; Kawashima, Hiroto ; Zhou, Bin ; Vestweber, Dietmar ; Red-Horse, Kristy ; Mebius, Reina E. ; Adams, Ralf H. ; Kubes, Paul ; Pan, Junliang ; Butcher, Eugene C. / A molecular map of murine lymph node blood vascular endothelium at single cell resolution. In: Nature Communications. 2020 ; Vol. 11, No. 1.

RIS

TY - JOUR

T1 - A molecular map of murine lymph node blood vascular endothelium at single cell resolution

AU - Brulois, Kevin

AU - Rajaraman, Anusha

AU - Szade, Agata

AU - Nordling, Sofia

AU - Bogoslowski, Ania

AU - Dermadi, Denis

AU - Rahman, Milladur

AU - Kiefel, Helena

AU - O’Hara, Edward

AU - Koning, Jasper J.

AU - Kawashima, Hiroto

AU - Zhou, Bin

AU - Vestweber, Dietmar

AU - Red-Horse, Kristy

AU - Mebius, Reina E.

AU - Adams, Ralf H.

AU - Kubes, Paul

AU - Pan, Junliang

AU - Butcher, Eugene C.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.

AB - Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.

UR - http://www.scopus.com/inward/record.url?scp=85088791783&partnerID=8YFLogxK

U2 - 10.1038/s41467-020-17291-5

DO - 10.1038/s41467-020-17291-5

M3 - Article

C2 - 32732867

AN - SCOPUS:85088791783

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3798

ER -