A multiplex label-free approach to avian influenza surveillance and serology

Research output: Contribution to journalArticle

Abstract

Influenza serology has traditionally relied on techniques such as hemagglutination inhibition, microneutralization, and ELISA. These assays are complex, challenging to implement in a format allowing detection of several types of antibody-analyte interactions at once (multiplex), and troublesome to implement in the field. As an alternative, we have developed a hemagglutinin microarray on the Arrayed Imaging Reflectometry (AIR) platform. AIR provides sensitive, rapid, and label-free multiplex detection of targets in complex analyte samples such as serum. In preliminary work, we demonstrated the application of this array to the testing of human samples from a vaccine trial. Here, we report the application of an expanded label-free hemagglutinin microarray to the analysis of avian serum samples. Samples from influenza virus challenge experiments in mallards yielded strong, selective detection of antibodies to the challenge antigen in most cases. Samples acquired in the field from mallards were also analyzed, and compared with viral hemagglutinin inhibition and microneutralization assays. We find that the AIR hemagglutinin microarray can provide a simple and robust alternative to standard methods, offering substantially greater information density from a simple workflow.

Details

Authors
  • Joseph Bucukovski
  • Neus Latorre-Margalef
  • David E. Stallknecht
  • Benjamin L. Miller
External organisations
  • University of Rochester
  • University of Georgia
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Zoology
Original languageEnglish
Article numbere0134484
JournalPLoS ONE
Volume10
Issue number8
Publication statusPublished - 2015 Aug 4
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes