A multi-purpose imaging endstation for high-resolution micrometer-scaled sub-second tomography

Research output: Contribution to journalArticle

Abstract

Time-resolved imaging of dynamic processes, ranging from biological in vivo studies to materials under in situ and in operando conditions, requires a flexible endstation capable of controlling complex components that interact in different configurations and at high speeds. At the X02DA TOMCAT beamline we have recently achieved in situ tomographic measurements at a rate of 20 Hz. Independently, we have shown the feasibility of in vivo lung imaging down to the micrometer scale. In the present paper, we discuss the latest developments in view of instrumentation and the accompanying components for achieving these two types of measurements. As the prime example, we focus on the technical requirements for in vivo tomographic microscopy of the lung at the micrometer scale in terms of acquisition schemes, triggering and radiation dose. We identify ultra-short single-projection exposures combined with accurate triggering capabilities as the main prerequisites to obtain high-quality reconstructions while limiting the X-ray dose imparted on the living sample. The presented endstation offers generic high-speed imaging capabilities, as it is compatible with a variety of experimental setups and suitable for a wide range of time-resolved studies.

Details

Authors
Organisations
External organisations
  • Paul Scherrer Institute
  • ETH Zürich
  • Swiss Federal Institute of Technology
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Radiology, Nuclear Medicine and Medical Imaging

Keywords

  • In vivo, Time-resolved tomography 2010, X-ray imaging
Original languageEnglish
Pages (from-to)1771-1778
Number of pages8
JournalPhysica Medica
Volume32
Issue number12
Publication statusPublished - 2016 Dec 1
Publication categoryResearch
Peer-reviewedYes