A novel model of dormancy for bone metastatic breast cancer cells

Research output: Contribution to journalArticle

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A novel model of dormancy for bone metastatic breast cancer cells. / Marlow, Rebecca; Honeth, Gabriella; Lombardi, Sara; Cariati, Massimiliano; Hessey, Sonya; Pipili, Aikaterini; Mariotti, Veronica; Buchupalli, Bharath; Foster, Katie; Bonnet, Dominique; Grigoriadis, Agamemnon; Rameshwar, Pranela; Purushotham, Anand; Tutt, Andrew; Dontu, Gabriela.

In: Cancer Research, Vol. 73, No. 23, 01.12.2013, p. 6886-99.

Research output: Contribution to journalArticle

Harvard

Marlow, R, Honeth, G, Lombardi, S, Cariati, M, Hessey, S, Pipili, A, Mariotti, V, Buchupalli, B, Foster, K, Bonnet, D, Grigoriadis, A, Rameshwar, P, Purushotham, A, Tutt, A & Dontu, G 2013, 'A novel model of dormancy for bone metastatic breast cancer cells', Cancer Research, vol. 73, no. 23, pp. 6886-99. https://doi.org/10.1158/0008-5472.CAN-13-0991

APA

Marlow, R., Honeth, G., Lombardi, S., Cariati, M., Hessey, S., Pipili, A., ... Dontu, G. (2013). A novel model of dormancy for bone metastatic breast cancer cells. Cancer Research, 73(23), 6886-99. https://doi.org/10.1158/0008-5472.CAN-13-0991

CBE

Marlow R, Honeth G, Lombardi S, Cariati M, Hessey S, Pipili A, Mariotti V, Buchupalli B, Foster K, Bonnet D, Grigoriadis A, Rameshwar P, Purushotham A, Tutt A, Dontu G. 2013. A novel model of dormancy for bone metastatic breast cancer cells. Cancer Research. 73(23):6886-99. https://doi.org/10.1158/0008-5472.CAN-13-0991

MLA

Vancouver

Marlow R, Honeth G, Lombardi S, Cariati M, Hessey S, Pipili A et al. A novel model of dormancy for bone metastatic breast cancer cells. Cancer Research. 2013 Dec 1;73(23):6886-99. https://doi.org/10.1158/0008-5472.CAN-13-0991

Author

Marlow, Rebecca ; Honeth, Gabriella ; Lombardi, Sara ; Cariati, Massimiliano ; Hessey, Sonya ; Pipili, Aikaterini ; Mariotti, Veronica ; Buchupalli, Bharath ; Foster, Katie ; Bonnet, Dominique ; Grigoriadis, Agamemnon ; Rameshwar, Pranela ; Purushotham, Anand ; Tutt, Andrew ; Dontu, Gabriela. / A novel model of dormancy for bone metastatic breast cancer cells. In: Cancer Research. 2013 ; Vol. 73, No. 23. pp. 6886-99.

RIS

TY - JOUR

T1 - A novel model of dormancy for bone metastatic breast cancer cells

AU - Marlow, Rebecca

AU - Honeth, Gabriella

AU - Lombardi, Sara

AU - Cariati, Massimiliano

AU - Hessey, Sonya

AU - Pipili, Aikaterini

AU - Mariotti, Veronica

AU - Buchupalli, Bharath

AU - Foster, Katie

AU - Bonnet, Dominique

AU - Grigoriadis, Agamemnon

AU - Rameshwar, Pranela

AU - Purushotham, Anand

AU - Tutt, Andrew

AU - Dontu, Gabriela

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Mortality of patients with breast cancer is due overwhelmingly to metastatic spread of the disease. Although dissemination is an early event in breast cancer, extended periods of cancer cell dormancy can result in long latency of metastasis development. Deciphering the mechanisms underlying cancer cell dormancy and subsequent growth at the metastatic site would facilitate development of strategies to interfere with these processes. A challenge in this undertaking has been the lack of models for cancer cell dormancy. We have established novel experimental systems that model the bone microenvironment of the breast cancer metastatic niche. These systems are based on 3D cocultures of breast cancer cells with cell types predominant in bone marrow. We identified conditions in which cancer cells are dormant and conditions in which they proliferate. Dormant cancer cells were able to proliferate upon transfer into supportive microenvironment or upon manipulation of signaling pathways that control dormancy. These experimental systems will be instrumental for metastasis studies, particularly the study of cellular dormancy.

AB - Mortality of patients with breast cancer is due overwhelmingly to metastatic spread of the disease. Although dissemination is an early event in breast cancer, extended periods of cancer cell dormancy can result in long latency of metastasis development. Deciphering the mechanisms underlying cancer cell dormancy and subsequent growth at the metastatic site would facilitate development of strategies to interfere with these processes. A challenge in this undertaking has been the lack of models for cancer cell dormancy. We have established novel experimental systems that model the bone microenvironment of the breast cancer metastatic niche. These systems are based on 3D cocultures of breast cancer cells with cell types predominant in bone marrow. We identified conditions in which cancer cells are dormant and conditions in which they proliferate. Dormant cancer cells were able to proliferate upon transfer into supportive microenvironment or upon manipulation of signaling pathways that control dormancy. These experimental systems will be instrumental for metastasis studies, particularly the study of cellular dormancy.

KW - Animals

KW - Bone Marrow Cells

KW - Bone Neoplasms

KW - Breast Neoplasms

KW - Cell Cycle Checkpoints

KW - Cells, Cultured

KW - Female

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - MCF-7 Cells

KW - Mice

KW - Mice, Inbred NOD

KW - Mice, SCID

KW - Models, Biological

KW - Neoplastic Stem Cells

KW - Stem Cell Niche

KW - Stromal Cells

KW - Tumor Microenvironment

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1158/0008-5472.CAN-13-0991

DO - 10.1158/0008-5472.CAN-13-0991

M3 - Article

VL - 73

SP - 6886

EP - 6899

JO - Cancer research. Supplement

T2 - Cancer research. Supplement

JF - Cancer research. Supplement

SN - 1538-7445

IS - 23

ER -