A novel procedure for the isolation of glycolipids from Bifidobacterium adolescentis 94 BIM using supercritical carbon dioxide

Research output: Contribution to journalArticle

Abstract

This study describes a novel isolation procedure for major glycolipids from Bifidobacterium adolescentis 94 BIM. The procedure consists of the use of supercritical carbon dioxide (scCO(2)) with hydro-methanolic solution as co-solvent. The major Glycolipids were isolated using the following operating conditions: pressure, 30 MPa, co-solvent concentration, 10% (9:1, methanol/water, v/v), CO2 flow rate, 5 g/min, extraction time and temperature, 2 h and 55 degrees C, respectively. The reference glycolipids sample was prepared by classical organic solvent extraction followed by chromatographic purification. All isolates were characterized by TLC and the major glycolipids additionally by enzyme linked immunosorbent (ELISA). Sixty milligrams of glycolipids with similar immunoreactivity as the reference glycolipids were isolated from I a of freeze-dried biomass (6% of yield). (c) 2005 Elsevier B.V. All rights reserved.

Details

Authors
  • Galina Novik
  • A Gamian
  • José da Cruz Francisco
  • Estera Dey
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • immunosorbent assay (ELISA), enzyme linked, Bifidobacterium adolescentis, bifidobacteria, neutral lipids, supercritical carbon dioxide, glycolipids
Original languageEnglish
Pages (from-to)555-562
JournalJournal of Biotechnology
Volume121
Issue number4
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes