A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

Research output: Contribution to journalArticle

Abstract

The peptide GSFSIQYTYHV derived from human semenogelin I forms a transparent hydrogel through spontaneous self-assembly in water at neutral pH. Linear rheology measurements demonstrate that the gel shows a dominating elastic response over a large frequency interval. CD, fluorescence and FTIR spectroscopy and cryo-TEM studies imply long fibrillar aggregates of extended β-sheet. Dynamic light scattering data indicate that the fibril lengths are of the order of micrometers. Time-dependent thioflavin T fluorescence shows that fibril formation by GSFSIQYTYHV is a nucleated reaction. The peptide may serve as basis for development of smart biomaterials of low immunogenicity suitable for biomedical applications, including drug delivery and wound healing.

Details

Authors
  • Birgitta Frohm
  • J E DeNizio
  • D S M Lee
  • L Gentile
  • U Olsson
  • Johan Malm
  • K S Akerfeldt
  • S Linse
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
Original languageEnglish
Pages (from-to)414-421
JournalSoft Matter
Volume11
Issue number2
Publication statusPublished - 2015
Publication categoryResearch
Peer-reviewedYes