A plant thiolase involved in benzoic acid biosynthesis and volatile benzenoid production

Research output: Contribution to journalArticle

Abstract

The exact biosynthetic pathways leading to benzoic acid (BA) formation in plants are not known, but labeling experiments indicate the contribution of both beta-oxidative and non-beta-oxidative pathways. In Petunia hybrida BA is a key precursor for the production of volatile benzenoids by its flowers. Using functional genomics, we identified a 3-ketoacyl-CoA thiolase, PhKAT1, which is involved in the benzenoid biosynthetic pathway and the production of BA. PhKAT1 is localised in the peroxisomes, where it is important for the formation of benzoyl-CoA-related compounds. Silencing of PhKAT1 resulted in a major reduction in BA and benzenoid formation, leaving the production of other phenylpropanoid-related volatiles unaffected. During the night, when volatile benzenoid production is highest, it is largely the beta-oxidative pathway that contributes to the formation of BA and benzenoids. Our studies add the benzenoid biosynthetic pathway to the list of pathways in which 3-ketoacyl-CoA thiolases are involved in plants.

Details

Authors
External organisations
  • University of Amsterdam
  • University of Michigan
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • Acetyl-CoA C-Acyltransferase, Benzoic Acid, DNA, Complementary, Flowers, Gene Expression Regulation, Plant, Gene Silencing, Molecular Sequence Data, Oils, Volatile, Peroxisomes, Petunia, Plant Proteins, Plants, Genetically Modified, RNA, Plant
Original languageEnglish
Pages (from-to)292-302
Number of pages11
JournalPlant Journal
Volume60
Issue number2
Publication statusPublished - 2009 Oct
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes