A porous silicon immunoassay platform for fluorometric determination of alpha-synuclein in human cerebrospinal fluid

Research output: Contribution to journalArticle

Abstract

Levels of total and/or oligomeric alpha-synuclein may be used as a biomarker tool to aid in the diagnosis and development of new disease-modifying therapies. We report here on a porous silicon antibody microarray for the fluorimetric determination of cerebrospinal fluid levels of total alpha-synuclein, a protein involved the pathology of Parkinson's disease. The surface of porous silicon has a 3-dimensional macro- and nanoporous structure, and this offers a large binding capacity for capturing probe molecules. Porous silicon also warrants efficient immobilization of antibodies by surface adsorption, and does not require chemical immobilization. The platform requires 10 mu L of cerebrospinal fluid, and each test requires 4 h for assay only (including immobilization of capturing antibody). The limit of detection is 35 pg mL(-1) of alpha-synuclein in cerebrospinal fluid, and the dynamic analytical range extends from 0.01 to 100 ng center dot mL(-1).

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Neurosciences
  • Neurology

Keywords

  • Porous silicon, Antibody microarray, alpha-synuclein, Parkinson's, disease
Original languageEnglish
Pages (from-to)1143-1149
JournalMikrochimica Acta
Volume181
Issue number9-10
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes