A potentiometric enzyme electrode for monitoring in organic solvents

Research output: Contribution to journalArticle

Abstract

A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology
Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalAnalytica Chimica Acta
Volume219
Issue numberC
Publication statusPublished - 1989 Jan 1
Publication categoryResearch
Peer-reviewedYes