A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.

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Abstract

We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment.

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Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • Antigen, T-Cell, gamma-delta/metabolism, Receptors, Interleukin-2/*genetics/*metabolism, alpha-beta/metabolism, Transgenic, Mice, Membrane Glycoproteins/*genetics, Liver/cytology/immunology, Hematopoietic Stem Cells/immunology, Genetic Markers, Reporter, Genes, Gene Expression, Fetus/cytology/immunology, B-Lymphocytes/immunology, CD44/metabolism, Animal, Antigens, T-Lymphocyte Subsets/*immunology, Thymus Gland/cytology/immunology
Original languageEnglish
Pages (from-to)119-128
JournalScandinavian Journal of Immunology
Volume55
Issue number2
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes