A simple and fast liquid-liquid extraction method for the determination of 2,2 ',4,4 ',5,5 '-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p '-DDE) from human serum for epidemiological studies on type 2 diabetes

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T1 - A simple and fast liquid-liquid extraction method for the determination of 2,2 ',4,4 ',5,5 '-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p '-DDE) from human serum for epidemiological studies on type 2 diabetes

AU - Rantakokko, Panu

AU - Kiviranta, Hannu

AU - Rylander, Lars

AU - Rignell-Hydbom, Anna

AU - Vartiainen, Terttu

PY - 2009

Y1 - 2009

N2 - A simple and fast method is presented to be used for example in studies on the relationship between serum levels of persistent organic pollutants and type 2 diabetes mellitus. Method is based on liquid-liquid extraction and gas chromatography coupled with high.-resolution mass spectrometry. In the sample pretreatment special attention was paid to minimize the number of sample manipulation steps and the amounts of organic solvents needed. Compounds analyzed were 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153) and 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE), the major metabolite of DDT. The method included extraction and cleanup of 0.2 rill of serum in a single test tube and subsequent analysis of the extract from 0.2 ml final volume. Validation was conducted to explore the performance of the method. The limits of detection for p,p'-DDE and PCB-153 based on the standard deviation of the blank samples were 4.3 and 3.1 pg/ml, respectively. Repeatability was less than 2.5% at three concentration levels tested and recovery from Certified Reference Material SRM 1589a was 84% for p,p'-DDE and 87% for PCB-153 of the certified values, respectively. Serum samples from the AMAP intercalibration round 2008-2 were also analyzed, and results were 101-116% of the assigned values. The presented method was used for an epidemiological study with more than 700 serum samples from a type 2 diabetes cohort from Sweden. (C) 2008 Elsevier B.V. All rights reserved.

AB - A simple and fast method is presented to be used for example in studies on the relationship between serum levels of persistent organic pollutants and type 2 diabetes mellitus. Method is based on liquid-liquid extraction and gas chromatography coupled with high.-resolution mass spectrometry. In the sample pretreatment special attention was paid to minimize the number of sample manipulation steps and the amounts of organic solvents needed. Compounds analyzed were 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153) and 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE), the major metabolite of DDT. The method included extraction and cleanup of 0.2 rill of serum in a single test tube and subsequent analysis of the extract from 0.2 ml final volume. Validation was conducted to explore the performance of the method. The limits of detection for p,p'-DDE and PCB-153 based on the standard deviation of the blank samples were 4.3 and 3.1 pg/ml, respectively. Repeatability was less than 2.5% at three concentration levels tested and recovery from Certified Reference Material SRM 1589a was 84% for p,p'-DDE and 87% for PCB-153 of the certified values, respectively. Serum samples from the AMAP intercalibration round 2008-2 were also analyzed, and results were 101-116% of the assigned values. The presented method was used for an epidemiological study with more than 700 serum samples from a type 2 diabetes cohort from Sweden. (C) 2008 Elsevier B.V. All rights reserved.

KW - PCB-153

KW - p

KW - p '-DDE

KW - organic pollutants (POPs)

KW - Persistent

KW - Type 2 diabetes

KW - Liquid-liquid extraction

KW - Human serum

U2 - 10.1016/j.chroma.2008.12.013

DO - 10.1016/j.chroma.2008.12.013

M3 - Article

VL - 1216

SP - 897

EP - 901

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 6

ER -