A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

A rapid and simple approach of lactose analysis is proposed based on 3rd generation amperometric biosensors employing cellobiose dehydrogenase (CDH) from Trametes villosa or Phanerochaete sordida immobilised on screen-printed carbon electrodes (SPCEs). After optimisation of the working conditions of the biosensors - pH of the carrier buffer, flow rate and applied potential - the sensors were able to detect lactose in a concentration range between 0.5-200 mu M and 0.5-100 mu M employing T. villosa and P. sordida CDH, respectively. The limit of detection is 250 nM (90 mu g/L) for both. Biosensors based on SPCEs modified with multiwalled carbon nanotubes showed a higher sensitivity than unmodified SPCEs. Crosslinking with glutaraldehyde or poly(ethyleneglycol)diglycidyl ether improved not only the stability but also the analytical response. The developed sensor has been successfully applied for the determination of lactose in dairy (milk with different percentages of fat, lactose-free milk and yogurt) with a good reproducibility (RSD = 1.5-2.2%). No sample preparation except a simple dilution process is needed. The biosensor is easy to make and operate, is inexpensive and reveals a high sensitivity and reliability. (C) 2009 Elsevier Ltd. All rights reserved.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Multiwalled carbon nanotubes, Lactose detection, Third generation biosensor, Cross-linking, Screen-printed electrodes
Original languageEnglish
Title of host publicationElectrochimica Acta
PublisherElsevier
Pages7690-7695
Volume55
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes
Event7th Spring Meeting of the International-Society-of-Electrochemistry on Recent Advances in Electrocatalysis and Photoelectrocatalysis - Szczyrk, Poland
Duration: 2009 Mar 222009 Mar 25

Publication series

Name
Number26
Volume55
ISSN (Print)0013-4686
ISSN (Electronic)1873-3859

Conference

Conference7th Spring Meeting of the International-Society-of-Electrochemistry on Recent Advances in Electrocatalysis and Photoelectrocatalysis
CountryPoland
CitySzczyrk
Period2009/03/222009/03/25