A tailored biocompatible neural interface for long term monitoring in neural networks

Research output: ThesisDoctoral Thesis (compilation)

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A tailored biocompatible neural interface for long term monitoring in neural networks. / Köhler, Per.

Neuronano Research Center (NRC), 2016. 76 p.

Research output: ThesisDoctoral Thesis (compilation)

Harvard

APA

CBE

Köhler P. 2016. A tailored biocompatible neural interface for long term monitoring in neural networks. Neuronano Research Center (NRC). 76 p.

MLA

Vancouver

Köhler P. A tailored biocompatible neural interface for long term monitoring in neural networks. Neuronano Research Center (NRC), 2016. 76 p. (Lund University Faculty of Medicine Doctoral Dissertation Series ).

Author

Köhler, Per. / A tailored biocompatible neural interface for long term monitoring in neural networks. Neuronano Research Center (NRC), 2016. 76 p.

RIS

TY - THES

T1 - A tailored biocompatible neural interface for long term monitoring in neural networks

AU - Köhler, Per

N1 - Defence details Date: 2016-03-04 Time: 09:00 Place: Belfragesalen, Klinikgatan 32, BMC D15, Lund. External reviewer(s) Name: Stieglitz, Thomas Title: [unknown] Affiliation: Universität Freiburg ---

PY - 2016

Y1 - 2016

N2 - Neural interface electrodes that can record from neurons in the brain for long periods of time will be of great importance to unravel how the brain accomplishes its functions. However, current electrodes usually cause significant glia reactions and loss of neurons within the adjacent brain parenchyma. To address this challenge, a novel, polymer-based neural probe, with protrusions tailored to the target tissue, was developed to investigate which probe properties affect the development of a glial scar and neuronal cell death surrounding probes. After many cycles of testing – refinements, promising recordings of neural activity were obtained in both cerebellum and cortex cerebri (papers I-III). In paper IV, we evaluated the importance of mechanical flexibility and demonstrated that probe flexibility has a significant impact on the astroglial scar, but not on the loss of neurons nearby. Moreover, by embedding the dummy probes in a gelatin matrix that dissolves shortly following implantation, neuronal cell death surrounding chronically (6 weeks) implanted electrodes was, for the first time, abolished. In paper V, sensory processing in primary somatosensory cortex during an episode of hyperalgesia was monitored using implanted neural interfaces in order to further evaluate the probe functionality and usefulness in neurophysiological research. By tracking the development of primary and secondary hyperalgesia as well as allodynia in the sensory cortex, we demonstrate the usefulness of our new neural interface and its capability to differentially and simultaneously record neural signals in different cortical laminae in awake freely moving animals.

AB - Neural interface electrodes that can record from neurons in the brain for long periods of time will be of great importance to unravel how the brain accomplishes its functions. However, current electrodes usually cause significant glia reactions and loss of neurons within the adjacent brain parenchyma. To address this challenge, a novel, polymer-based neural probe, with protrusions tailored to the target tissue, was developed to investigate which probe properties affect the development of a glial scar and neuronal cell death surrounding probes. After many cycles of testing – refinements, promising recordings of neural activity were obtained in both cerebellum and cortex cerebri (papers I-III). In paper IV, we evaluated the importance of mechanical flexibility and demonstrated that probe flexibility has a significant impact on the astroglial scar, but not on the loss of neurons nearby. Moreover, by embedding the dummy probes in a gelatin matrix that dissolves shortly following implantation, neuronal cell death surrounding chronically (6 weeks) implanted electrodes was, for the first time, abolished. In paper V, sensory processing in primary somatosensory cortex during an episode of hyperalgesia was monitored using implanted neural interfaces in order to further evaluate the probe functionality and usefulness in neurophysiological research. By tracking the development of primary and secondary hyperalgesia as well as allodynia in the sensory cortex, we demonstrate the usefulness of our new neural interface and its capability to differentially and simultaneously record neural signals in different cortical laminae in awake freely moving animals.

KW - Brain-machine interfaces

KW - neuroinflammation

KW - somatosensory cortex

KW - hyperalgesia

KW - allodynia

M3 - Doctoral Thesis (compilation)

SN - 978-91-7619-248-1

T3 - Lund University Faculty of Medicine Doctoral Dissertation Series

PB - Neuronano Research Center (NRC)

ER -