A von Willebrand factor-binding protein from Staphylococcus lugdunensis

Research output: Contribution to journalArticle

Abstract

In the present study, a phage display library covering the genome of Staphylococcus lugdunensis, was affinity-selected against von Willebrand factor (vWf). This led to the identification of a gene, vwbl, encoding a Putative cell surface protein of 2060 amino acids, denoted vWbl. The deduced protein has an overall organisation typical of staphylococcal cell Surface proteins, with an N-terminal signal peptide, and a C-terminal cell wall sorting signal. The vWf-binding part is located in repetitive domains and antibodies against vWbl or vWf can inhibit the binding. Southern blot analysis showed that vwbl was present in the 12 S. lugdunensis strains tested. (C) 2004 Federation of European Microbiological Societies.

Details

Authors
  • M Nilsson
  • J Bjerketorp
  • A Wiebensjo
  • Åsa Ljungh
  • L Frykberg
  • B Guss
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area

Keywords

  • von Willebrand factor, display, phage, coagulase-negative staphylococci, Staphylococcus lugdunensis, adhesin, LPXTG motif
Original languageEnglish
Pages (from-to)155-161
JournalFEMS Microbiology Letters
Volume234
Issue number1
Publication statusPublished - 2004
Publication categoryResearch
Peer-reviewedYes