Abnormal angiogenesis but intact hematopoietic potential in TGF-beta type I receptor-deficient mice

Research output: Contribution to journalArticle

Abstract

Deletion of the transforming growth factor beta1 (TGF-beta1) gene in mice has previously suggested that it regulates both hematopoiesis and angiogenesis. To define the function of TGF-beta more precisely, we inactivated the TGF-beta type I receptor (T beta RI) gene by gene targeting. Mice lacking T beta RI die at midgestation, exhibiting severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells. However, despite obvious anemia in the T beta RI-/- yolk sacs, clonogenic assays on yolk sac-derived hematopoietic precursors in vitro revealed that T beta RI-/- mice exhibit normal hematopoietic potential compared with wild-type and heterozygous siblings, Endothelial cells derived from T beta RI-deficient embryos show enhanced cell proliferation, improper migratory behavior and impaired fibronectin production in vitro, defects that are associated with the vascular defects seen in vivo. We thus demonstrate here that, while T beta RI is crucial for the function of TGF-beta during vascular development and can not be compensated for by the activin receptor-like kinase-1 (ALK-1), functional hematopoiesis and development of hematopoietic progenitors is not dependent on TGF-beta signaling via T beta RI.

Details

Authors
  • Jonas Larsson
  • Marie-José Goumans
  • Lottie Jansson Sjöstrand
  • Marga A. van Rooijen
  • Dorien Ward
  • Per Levéen
  • Xiufeng Xu
  • Peter ten Dijke
  • Christine L. Mummery
  • Stefan Karlsson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • endothelial cell, hematopoiesis, serine, signal transduction, TGF-beta, threonine kinase receptor
Original languageEnglish
Pages (from-to)1663-1673
JournalEMBO Journal
Volume20
Issue number7
Publication statusPublished - 2001
Publication categoryResearch
Peer-reviewedYes