Absence of Epstein-Barr and Cytomegalovirus Infection in Neuroblastoma Cells by Standard Detection Methodologies.

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Abstract

Indications exist in the scientific literature that infection with human herpes family viruses may contribute to the pathogenesis of neuroblastoma (NB). However, systematic investigations regarding viral presence in NB cells have been scarcely reported. Here, the presence of DNA from Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by PCR in 12 NBs, supplemented with RNA in situ hybridization, immunohistochemical detection, and high-throughput DNA sequencing. These standard methods did not detect infection by EBV or HCMV in NB cells in any tumor, while occasional immune cells were positive for EBV RNA or HCMV protein in four cases. Pediatr Blood Cancer © 2013 Wiley Periodicals, Inc.

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  • Pediatrics
Original languageEnglish
Pages (from-to)E91-E93
JournalPediatric Blood & Cancer
Volume60
Issue number9
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes