Absence of Epstein-Barr and Cytomegalovirus Infection in Neuroblastoma Cells by Standard Detection Methodologies.

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Absence of Epstein-Barr and Cytomegalovirus Infection in Neuroblastoma Cells by Standard Detection Methodologies. / Sehic, Daniel; Forslund, Ola; Sandén, Emma; Holmquist Mengelbier, Linda; Karlsson, Jenny; Bzhalava, Davit; Ekström, Johanna; Warenholt, Janina; Darabi, Anna; Dillner, Joakim; Øra, Ingrid; Gisselsson Nord, David.

In: Pediatric Blood & Cancer, Vol. 60, No. 9, 2013, p. E91-E93.

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TY - JOUR

T1 - Absence of Epstein-Barr and Cytomegalovirus Infection in Neuroblastoma Cells by Standard Detection Methodologies.

AU - Sehic, Daniel

AU - Forslund, Ola

AU - Sandén, Emma

AU - Holmquist Mengelbier, Linda

AU - Karlsson, Jenny

AU - Bzhalava, Davit

AU - Ekström, Johanna

AU - Warenholt, Janina

AU - Darabi, Anna

AU - Dillner, Joakim

AU - Øra, Ingrid

AU - Gisselsson Nord, David

PY - 2013

Y1 - 2013

N2 - Indications exist in the scientific literature that infection with human herpes family viruses may contribute to the pathogenesis of neuroblastoma (NB). However, systematic investigations regarding viral presence in NB cells have been scarcely reported. Here, the presence of DNA from Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by PCR in 12 NBs, supplemented with RNA in situ hybridization, immunohistochemical detection, and high-throughput DNA sequencing. These standard methods did not detect infection by EBV or HCMV in NB cells in any tumor, while occasional immune cells were positive for EBV RNA or HCMV protein in four cases. Pediatr Blood Cancer © 2013 Wiley Periodicals, Inc.

AB - Indications exist in the scientific literature that infection with human herpes family viruses may contribute to the pathogenesis of neuroblastoma (NB). However, systematic investigations regarding viral presence in NB cells have been scarcely reported. Here, the presence of DNA from Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by PCR in 12 NBs, supplemented with RNA in situ hybridization, immunohistochemical detection, and high-throughput DNA sequencing. These standard methods did not detect infection by EBV or HCMV in NB cells in any tumor, while occasional immune cells were positive for EBV RNA or HCMV protein in four cases. Pediatr Blood Cancer © 2013 Wiley Periodicals, Inc.

U2 - 10.1002/pbc.24535

DO - 10.1002/pbc.24535

M3 - Article

VL - 60

SP - E91-E93

JO - Pediatric Blood & Cancer

JF - Pediatric Blood & Cancer

SN - 1545-5017

IS - 9

ER -