Acoustic separation of bacteria from blood cells at high cell concentrations enabled by acoustic impedance matched buffers

Research output: Chapter in Book/Report/Conference proceedingPaper in conference proceeding

Abstract

We have developed a method to separate bacteria from red and white blood cells (henceforth collectively called blood cells) using acoustophoresis, for subsequent PCR detection. To maximize throughput, we have now investigated the effect of increased cell concentration on the separation. Increasing the blood concentration drastically decreases bacteria recovery, but we show that this can be remedied by optimizing the acoustic impedance of the center buffer. This way we can recover 89% of the bacteria while removing more than 99.8% of the blood cells from 1 ml whole blood within 25 min, paving the way for new sepsis diagnostics methods.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biomedical Laboratory Science/Technology

Keywords

  • Acoustophoresis, Bacteria, Blood, Sepsis
Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages388-390
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30
Conference number: 18

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period2014/10/262014/10/30