Acoustofluidic harvesting of microalgae on a single chip

Research output: Contribution to journalArticle

Abstract

We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76, and a cell viability rate of 98.3% at a high volume rate of 500 μl/min. Our results indicate that the on-chip acoustofluidic platform is an effective tool for harvesting target microalgae from mixed populations of microalgae and other contaminants.

Details

Authors
  • Jee Woong Park
  • Soo Hyeon Kim
  • Takuro Ito
  • Teruo Fujii
  • So Youn Kim
  • Thomas Laurell
  • Sang Wook Lee
  • Keisuke Goda
Organisations
External organisations
  • University of Tokyo
  • Dongguk University, Seoul
  • Keio University
  • University of California, Los Angeles
  • Japan Science and Technology Agency (JST)
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology
Original languageEnglish
Article number034119
JournalBiomicrofluidics
Volume10
Issue number3
Publication statusPublished - 2016 May 1
Publication categoryResearch
Peer-reviewedYes