Acoustofluidic hematocrit determination

Research output: Contribution to journalArticle

Abstract

Hematocrit (HCT) measurements of blood from patients, blood donors and athletes are routinely performed on a daily basis. These measurements are often performed in centralized hospital labs by whole blood analyzers, which leads to long time-to-result. On site measurements, based on centrifugation can be done, but these assays require manual handling, are slow and can just measure HCT in contrast to the central lab whole blood analyzers. In this work, we present a microfluidic based method to measure HCT in blood samples by acoustic separation of whole blood into discrete regions of plasma and red blood cells. Comparison of the areas of the red blood cell and plasma regions gives an accurate HCT value, with a linear correlation to the centrifugation-based reference method. A readout can be performed within 2 s of acoustic actuation providing a readout accuracy of approximately 3% points (pp) HCT. Additional accuracy can be achieved by extending the acoustic actuation to 20 s, yielding an error of less than 1 pp HCT. This acoustic tool is optimal for integration into a lab-on-a-chip device with in-line measurements of different clinical parameters.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
  • Dongguk University, Seoul
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biomedical Laboratory Science/Technology

Keywords

  • Acoustofluidic, Acoustophoresis, Hematocrit, Microfluidic blood analyze
Original languageEnglish
Pages (from-to)199-204
JournalAnalytica Chimica Acta
Volume1000
Early online date2017 Nov 22
Publication statusPublished - 2018
Publication categoryResearch
Peer-reviewedYes

Related research output

Klara Petersson, 2018 Feb 20, 1 ed. Lund: Department of Biomedical Engineering, Lund university. 159 p.

Research output: ThesisDoctoral Thesis (compilation)

View all (1)