Acoustofluidic hematocrit determination

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Acoustofluidic hematocrit determination. / Petersson, Klara; Jakobsson, Ola; Ohlsson, Pelle; Augustsson, Per; Scheding, Stefan; Malm, Johan; Laurell, Thomas.

In: Analytica Chimica Acta, Vol. 1000, 2018, p. 199-204.

Research output: Contribution to journalArticle

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TY - JOUR

T1 - Acoustofluidic hematocrit determination

AU - Petersson, Klara

AU - Jakobsson, Ola

AU - Ohlsson, Pelle

AU - Augustsson, Per

AU - Scheding, Stefan

AU - Malm, Johan

AU - Laurell, Thomas

PY - 2018

Y1 - 2018

N2 - Hematocrit (HCT) measurements of blood from patients, blood donors and athletes are routinely performed on a daily basis. These measurements are often performed in centralized hospital labs by whole blood analyzers, which leads to long time-to-result. On site measurements, based on centrifugation can be done, but these assays require manual handling, are slow and can just measure HCT in contrast to the central lab whole blood analyzers. In this work, we present a microfluidic based method to measure HCT in blood samples by acoustic separation of whole blood into discrete regions of plasma and red blood cells. Comparison of the areas of the red blood cell and plasma regions gives an accurate HCT value, with a linear correlation to the centrifugation-based reference method. A readout can be performed within 2 s of acoustic actuation providing a readout accuracy of approximately 3% points (pp) HCT. Additional accuracy can be achieved by extending the acoustic actuation to 20 s, yielding an error of less than 1 pp HCT. This acoustic tool is optimal for integration into a lab-on-a-chip device with in-line measurements of different clinical parameters.

AB - Hematocrit (HCT) measurements of blood from patients, blood donors and athletes are routinely performed on a daily basis. These measurements are often performed in centralized hospital labs by whole blood analyzers, which leads to long time-to-result. On site measurements, based on centrifugation can be done, but these assays require manual handling, are slow and can just measure HCT in contrast to the central lab whole blood analyzers. In this work, we present a microfluidic based method to measure HCT in blood samples by acoustic separation of whole blood into discrete regions of plasma and red blood cells. Comparison of the areas of the red blood cell and plasma regions gives an accurate HCT value, with a linear correlation to the centrifugation-based reference method. A readout can be performed within 2 s of acoustic actuation providing a readout accuracy of approximately 3% points (pp) HCT. Additional accuracy can be achieved by extending the acoustic actuation to 20 s, yielding an error of less than 1 pp HCT. This acoustic tool is optimal for integration into a lab-on-a-chip device with in-line measurements of different clinical parameters.

KW - Acoustofluidic

KW - Acoustophoresis

KW - Hematocrit

KW - Microfluidic blood analyze

U2 - 10.1016/j.aca.2017.11.037

DO - 10.1016/j.aca.2017.11.037

M3 - Article

VL - 1000

SP - 199

EP - 204

JO - Analytica Chimica Acta

T2 - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 1873-4324

ER -