Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum

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title = "Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum",
abstract = "Background: Antibodies that inhibit the growth of blood-stage Plasmodium falciparum may play an important role in acquired and vaccine-induced immunity in humans. However, the acquisition and activity of these antibodies is not well understood. Methods: We tested dialysed serum and purified immunoglobulins from Kenyan children and adults for inhibition of P. falciparum blood-stage growth in vitro using different parasite lines. Serum antibodies were measured by ELISA to blood-stage parasite antigens, extracted from P. falciparum schizonts, and to recombinant merozoite surface protein 1 (42 kDa C-terminal fragment, MSP1-42). Results: Antibodies to blood-stage antigens present in schizont protein extract and to recombinant MSP1-42 significantly increased with age and were highly correlated. In contrast, growth-inhibitory activity was not strongly associated with age and tended to decline marginally with increasing age and exposure, with young children demonstrating the highest inhibitory activity. Comparison of growth-inhibitory activity among samples collected from the same population at different time points suggested that malaria transmission intensity influenced the level of growth-inhibitory antibodies. Antibodies to recombinant MSP1-42 were not associated with growth inhibition and high immunoglobulin G levels were poorly predictive of inhibitory activity. The level of inhibitory activity against different isolates varied. Conclusions: Children can acquire growth-inhibitory antibodies at a young age, but once they are acquired they do not appear to be boosted by on-going exposure. Inhibitory antibodies may play a role in protection from early childhood malaria.",
keywords = "recombinant merozoite surface protein 1, parasite antibody, merozoite surface protein 1, immunoglobulin G, unclassified drug, protozoon antibody",
author = "McCallum, {F. J.} and Kristina Persson and Mugyenyi, {C. K.} and Fowkes, {F. J. I.} and Simpson, {J. A.} and Richards, {J. S.} and Williams, {T. N.} and K. Marsh and Beeson, {J. G.}",
note = "10",
year = "2008",
doi = "10.1371/journal.pone.0003571",
language = "English",
volume = "3",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "10",