Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum

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Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum. / McCallum, F. J.; Persson, Kristina; Mugyenyi, C. K.; Fowkes, F. J. I.; Simpson, J. A.; Richards, J. S.; Williams, T. N.; Marsh, K.; Beeson, J. G.

In: PLoS ONE, Vol. 3, No. 10, e3571, 2008.

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Harvard

McCallum, FJ, Persson, K, Mugyenyi, CK, Fowkes, FJI, Simpson, JA, Richards, JS, Williams, TN, Marsh, K & Beeson, JG 2008, 'Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum', PLoS ONE, vol. 3, no. 10, e3571. https://doi.org/10.1371/journal.pone.0003571

APA

McCallum, F. J., Persson, K., Mugyenyi, C. K., Fowkes, F. J. I., Simpson, J. A., Richards, J. S., Williams, T. N., Marsh, K., & Beeson, J. G. (2008). Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum. PLoS ONE, 3(10), [e3571]. https://doi.org/10.1371/journal.pone.0003571

CBE

McCallum FJ, Persson K, Mugyenyi CK, Fowkes FJI, Simpson JA, Richards JS, Williams TN, Marsh K, Beeson JG. 2008. Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum. PLoS ONE. 3(10):Article e3571. https://doi.org/10.1371/journal.pone.0003571

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McCallum, F. J. ; Persson, Kristina ; Mugyenyi, C. K. ; Fowkes, F. J. I. ; Simpson, J. A. ; Richards, J. S. ; Williams, T. N. ; Marsh, K. ; Beeson, J. G. / Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum. In: PLoS ONE. 2008 ; Vol. 3, No. 10.

RIS

TY - JOUR

T1 - Acquisition of Growth-Inhibitory Antibodies against Blood-Stage Plasmodium falciparum

AU - McCallum, F. J.

AU - Persson, Kristina

AU - Mugyenyi, C. K.

AU - Fowkes, F. J. I.

AU - Simpson, J. A.

AU - Richards, J. S.

AU - Williams, T. N.

AU - Marsh, K.

AU - Beeson, J. G.

N1 - 10

PY - 2008

Y1 - 2008

N2 - Background: Antibodies that inhibit the growth of blood-stage Plasmodium falciparum may play an important role in acquired and vaccine-induced immunity in humans. However, the acquisition and activity of these antibodies is not well understood. Methods: We tested dialysed serum and purified immunoglobulins from Kenyan children and adults for inhibition of P. falciparum blood-stage growth in vitro using different parasite lines. Serum antibodies were measured by ELISA to blood-stage parasite antigens, extracted from P. falciparum schizonts, and to recombinant merozoite surface protein 1 (42 kDa C-terminal fragment, MSP1-42). Results: Antibodies to blood-stage antigens present in schizont protein extract and to recombinant MSP1-42 significantly increased with age and were highly correlated. In contrast, growth-inhibitory activity was not strongly associated with age and tended to decline marginally with increasing age and exposure, with young children demonstrating the highest inhibitory activity. Comparison of growth-inhibitory activity among samples collected from the same population at different time points suggested that malaria transmission intensity influenced the level of growth-inhibitory antibodies. Antibodies to recombinant MSP1-42 were not associated with growth inhibition and high immunoglobulin G levels were poorly predictive of inhibitory activity. The level of inhibitory activity against different isolates varied. Conclusions: Children can acquire growth-inhibitory antibodies at a young age, but once they are acquired they do not appear to be boosted by on-going exposure. Inhibitory antibodies may play a role in protection from early childhood malaria.

AB - Background: Antibodies that inhibit the growth of blood-stage Plasmodium falciparum may play an important role in acquired and vaccine-induced immunity in humans. However, the acquisition and activity of these antibodies is not well understood. Methods: We tested dialysed serum and purified immunoglobulins from Kenyan children and adults for inhibition of P. falciparum blood-stage growth in vitro using different parasite lines. Serum antibodies were measured by ELISA to blood-stage parasite antigens, extracted from P. falciparum schizonts, and to recombinant merozoite surface protein 1 (42 kDa C-terminal fragment, MSP1-42). Results: Antibodies to blood-stage antigens present in schizont protein extract and to recombinant MSP1-42 significantly increased with age and were highly correlated. In contrast, growth-inhibitory activity was not strongly associated with age and tended to decline marginally with increasing age and exposure, with young children demonstrating the highest inhibitory activity. Comparison of growth-inhibitory activity among samples collected from the same population at different time points suggested that malaria transmission intensity influenced the level of growth-inhibitory antibodies. Antibodies to recombinant MSP1-42 were not associated with growth inhibition and high immunoglobulin G levels were poorly predictive of inhibitory activity. The level of inhibitory activity against different isolates varied. Conclusions: Children can acquire growth-inhibitory antibodies at a young age, but once they are acquired they do not appear to be boosted by on-going exposure. Inhibitory antibodies may play a role in protection from early childhood malaria.

KW - recombinant merozoite surface protein 1

KW - parasite antibody

KW - merozoite surface protein 1

KW - immunoglobulin G

KW - unclassified drug

KW - protozoon antibody

U2 - 10.1371/journal.pone.0003571

DO - 10.1371/journal.pone.0003571

M3 - Article

VL - 3

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 10

M1 - e3571

ER -