Activation of enzymes in organic media at low water activity by polyols and saccharides

Research output: Contribution to journalArticle

Abstract

Horse liver alcohol dehydrogenase and α-chymotrypsin were deposited on a porous support material, Celite. After equilibration at a well-defined water activity, the catalytic activity was measured with diisopropyl ether as reaction medium. The effects of the presence of polyols and simple saccharides in the preparations were investigated. The additives caused a considerable increase in the amount of water bound to the preparation at a fixed water activity. At low water activities the catalytic activity was increased and at high water activity it was decreased by the additives. The presence of additives increased the ratio of alcoholysis-to-hydrolysis activity of chymotrypsin.

Details

Authors
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology

Keywords

  • Enzyme activation, Low water activity, Organic medium
Original languageEnglish
Pages (from-to)144-148
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1163
Issue number2
Publication statusPublished - 1993 May 13
Publication categoryResearch
Peer-reviewedYes