Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection

Research output: Contribution to journalArticle

Abstract

Fiber and penton base overproduced in adenovirus (Ad) infected cells can be secreted prior to progeny release and thereby regulate progeny spread. We aimed to investigate the mechanisms of fiber and penton base secretion in Ad2- or Ad5-infected A549 cells. Our flow cytometry analyses detected abundant surface fiber molecules, but little penton base molecules at 12 h post infection. Immunogold staining combined with transmission electron microscopic analyses revealed separate, non-co-localized release of fiber and penton base in the proximity of the plasma membrane. Depolymerization of microtubule and actin cytoskeletons, and inhibition of Rock kinase and myosin II activity together demonstrated cytoskeletal network-dependent fiber secretion. Inhibition of intracellular calcium [Ca2+]i signaling caused diminished fiber secretion, which was associated with diminished progeny production. Thus, fiber and penton base are actively and separately secreted during the early stages of Ad2 or Ad5 infection, their secretion may play important role in Ad life cycle.

Details

Authors
Organisations
External organisations
  • Beijing Normal University
  • Université Claude Bernard
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area

Keywords

  • Adenovirus, Fiber protein, Penton base, Secretion
Original languageEnglish
Pages (from-to)172-180
Number of pages9
JournalVirology
Volume505
Publication statusPublished - 2017 May 1
Publication categoryResearch
Peer-reviewedYes