Acute retinal ischemia caused by controlled low ocular perfusion pressure in a porcine model. Electrophysiological and histological characterisation

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to establish, and characterize a porcine model of acute, controlled retinal ischemia. The controlled retinal ischemia was produced by clamping the ocular perfusion pressure (OPP) in the left eye to 5 mm Hg for 2 h. The OPP was defined as mean arterial blood pressure (MAP) minus the intraocular pressure (IOP). It was clamped to 0-30 mm Hg by continuous monitoring of MAP and adjustment of the IOP, which was controlled by cannulation of the anterior chamber. Inner retinal function was assessed by induced multifocal electroretinography (mfERG) with comparisons of the amplitudes obtained in the experimental, left eye, and the control, right eye. Quantitative histology was performed to measure the survival of ganglion cells, amacrine cells and horizontal cells 2-6 weeks after the ischemic insult. An OPP of 5 mm Hg for 2 h induced significant reductions in the amplitudes of iN1 to 20% (CI: 13-30%), and iPr2 to 14% (95% CI: 8-22%) of their baseline values. No signs of recovery were found within the 6-week observation period. Quantitative histology revealed a highly significant reduction in the number of ganglion cells, amacrine cells and horizontal cells after the ischemic insult. This model seems to be suitable for investigations of therapeutic initiatives in diseases involving acute retinal ischemia. (C) 2009 Elsevier Ltd. All rights reserved.

Details

Authors
  • Maria Voss Kyhn
  • Karin Warfvinge
  • Erik Scherfig
  • Jens F. Kiilgaard
  • Jan Ulrik Prause
  • Henry Klassen
  • Michael Young
  • Morten la Cour
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ophthalmology

Keywords

  • perfusion pressure, low, retinal ischemia, induced components, pig, multifocal ERG, acute glaucoma, anaesthesia
Original languageEnglish
Pages (from-to)1100-1106
JournalExperimental Eye Research
Volume88
Issue number6
Publication statusPublished - 2009
Publication categoryResearch
Peer-reviewedYes