Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards

Research output: Contribution to journalArticle

Standard

Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards. / Segovia, Karen M.; Stallknecht, David E.; Kapczynski, Darrell R.; Stabler, Lisa; Berghaus, Roy D.; Fotjik, Alinde; Latorre-Margalef, Neus; França, Monique S.

In: PLoS ONE, Vol. 12, No. 1, e0170335, 01.01.2017.

Research output: Contribution to journalArticle

Harvard

Segovia, KM, Stallknecht, DE, Kapczynski, DR, Stabler, L, Berghaus, RD, Fotjik, A, Latorre-Margalef, N & França, MS 2017, 'Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards', PLoS ONE, vol. 12, no. 1, e0170335. https://doi.org/10.1371/journal.pone.0170335

APA

Segovia, K. M., Stallknecht, D. E., Kapczynski, D. R., Stabler, L., Berghaus, R. D., Fotjik, A., Latorre-Margalef, N., & França, M. S. (2017). Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards. PLoS ONE, 12(1), [e0170335]. https://doi.org/10.1371/journal.pone.0170335

CBE

Segovia KM, Stallknecht DE, Kapczynski DR, Stabler L, Berghaus RD, Fotjik A, Latorre-Margalef N, França MS. 2017. Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards. PLoS ONE. 12(1):Article e0170335. https://doi.org/10.1371/journal.pone.0170335

MLA

Vancouver

Segovia KM, Stallknecht DE, Kapczynski DR, Stabler L, Berghaus RD, Fotjik A et al. Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards. PLoS ONE. 2017 Jan 1;12(1). e0170335. https://doi.org/10.1371/journal.pone.0170335

Author

Segovia, Karen M. ; Stallknecht, David E. ; Kapczynski, Darrell R. ; Stabler, Lisa ; Berghaus, Roy D. ; Fotjik, Alinde ; Latorre-Margalef, Neus ; França, Monique S. / Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards. In: PLoS ONE. 2017 ; Vol. 12, No. 1.

RIS

TY - JOUR

T1 - Adaptive heterosubtypic immunity to low pathogenic avian influenza viruses in experimentally infected mallards

AU - Segovia, Karen M.

AU - Stallknecht, David E.

AU - Kapczynski, Darrell R.

AU - Stabler, Lisa

AU - Berghaus, Roy D.

AU - Fotjik, Alinde

AU - Latorre-Margalef, Neus

AU - França, Monique S.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Mallards are widely recognized as reservoirs for Influenza A viruses (IAV); however, host factors that might prompt seasonality and trends in subtype diversity of IAV such as adaptive heterosubtypic immunity (HSI) are not well understood. To investigate this, we inoculated mallards with a prevailing H3N8 low pathogenic avian influenza virus (LPAIV) subtype in waterfowl to determine if prior infection with this virus would be protective against heterosubtypic infections with the H4N6, H10N7 and H14N5 LPAIV subtypes after one, two and three months, respectively. Also, we investigated the effect of cumulative immunity after sequential inoculation of mallards with these viruses in one-month intervals. Humoral immunity was assessed by microneutralization assays using a subset of representative LPAIV subtypes as antigens. Our results indicate that prior inoculation with the H3N8 virus confers partial protective immunity against subsequent heterosubtypic infections with the robustness of HSI related to the phylogenetic similarity of the HA protein of the strains used. Furthermore, induced HSI was boosted and followed by repeated exposure to more than one LPAIV subtype. Our findings provide further information on the contributions of HSI and its role in the dynamics of IAV subtype diversity in mallards.

AB - Mallards are widely recognized as reservoirs for Influenza A viruses (IAV); however, host factors that might prompt seasonality and trends in subtype diversity of IAV such as adaptive heterosubtypic immunity (HSI) are not well understood. To investigate this, we inoculated mallards with a prevailing H3N8 low pathogenic avian influenza virus (LPAIV) subtype in waterfowl to determine if prior infection with this virus would be protective against heterosubtypic infections with the H4N6, H10N7 and H14N5 LPAIV subtypes after one, two and three months, respectively. Also, we investigated the effect of cumulative immunity after sequential inoculation of mallards with these viruses in one-month intervals. Humoral immunity was assessed by microneutralization assays using a subset of representative LPAIV subtypes as antigens. Our results indicate that prior inoculation with the H3N8 virus confers partial protective immunity against subsequent heterosubtypic infections with the robustness of HSI related to the phylogenetic similarity of the HA protein of the strains used. Furthermore, induced HSI was boosted and followed by repeated exposure to more than one LPAIV subtype. Our findings provide further information on the contributions of HSI and its role in the dynamics of IAV subtype diversity in mallards.

UR - http://www.scopus.com/inward/record.url?scp=85010451182&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0170335

DO - 10.1371/journal.pone.0170335

M3 - Article

C2 - 28107403

AN - SCOPUS:85010451182

VL - 12

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 1

M1 - e0170335

ER -