Adsorption from lipase-surfactant solutions onto methylated silica surfaces

Research output: Contribution to journalArticle

Abstract

In situ ellipsometry was used to study the adsorption/desorption of highly purified lipase from Humicola lanuginosa in mixtures with surfactants, at the solid/liquid interface. The effect of the surfactant was studied both when it was allowed to adsorb in mixture with lipase and when added after lipase adsorption. Silica surfaces, totally or partially methylated, were used and the surfactants were SDS (anionic), C12E5 and a commercial alcohol ethoxylate (AE) (both nonionic). The experiments were carried out so as to simulate a laundry process and the pH throughout the study was kept at 9 by means of Tris-HCl buffer.

From the results it was shown that the lipase did not adsorb until the diluted, that is during the rinsing period. This was found for all the lipase-surfactant mixtures in the study. However, the amount of lipase adsorbed was larger after rinsing in mixture with SDS, compared with C12E5. The results from addition of surfactant after lipase adsorption indicated that the lipase was replaced by surfactant. This was found for SDS and C12E5 at both the hydrophobic surface and the surface with intermediate hydrophobicity.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Physical Chemistry
  • Materials Chemistry
Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume6
Issue number1
Publication statusPublished - 1996
Publication categoryResearch
Peer-reviewedYes